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A fish cytokine related to human IL-3, IL-5, and GM-CSF, induces development of eosinophil/basophil/mast-cell type (EBM) granulocytes.

Interleukin-3 (IL-3), IL-5, and granulocyte-macrophage colony-stimulating issue (GM-CSF) are related cytokines that sign by way of receptors possessing the β frequent (βc) chain. As a household, these cytokines mix quite non-specific hematopoietic progress issue properties with a particular significance for eosinophils, basophils, and mast cells. In fish the cytokines of this household are known as IL-5fam, and the current examine, utilizing carp, constitutes their first useful evaluation.
Carp il-5fam expression was enhanced by stimulation with phytohemagglutinin and killed micro organism. Reminiscent of mammalian IL-3/IL-5/GM-CSF relations, recombinant carp IL-5fam (rcIL-5fam) induced activation of transcription issue STAT5 and effectively promoted proliferation and colony-formation of eosinophil/basophil/mast-cell type (EBM) granulocytes.
Upon addition of recombinant carp βc the expansion impact of rcIL-5fam was diminished, suggesting βc participation within the signaling route. In abstract, regardless of variations in particular person cytokines and cell populations, fish and mammalian IL-3/IL-5/GM-CSF relations share progress issue features for non-neutrophil granulocytes.

Clinical Physicians’ Attitudes in direction of Evidence-Based Medicine (EBM) and Their Evidence-Based Practice (EBP) in Wuhan, China.

Objective: Numerous research have proved the significance of Evidence-Based Medicine (EBM) in each day scientific observe, nevertheless, clinicians’ attitudes play an vital position in figuring out its implementation. The goal of this examine was to examine Chinese scientific physicians’ notion of and angle in direction of EBM and their Evidence-Based Practice (EBP) in addition to the obstacles in direction of EBP. Methods: Using a cross-sectional design, self-response questionnaires have been distributed to scientific physicians (inside medication and surgical procedure departments) throughout three tertiary hospitals in Wuhan, China. Results: In complete, 131 out of 195 (67.2%) physicians accomplished and returned the questionnaire.
A complete of 64.9% of the physicians both knew reasonably or rather a lot about EBM. The imply rating of physicians’ angle towards EBM was 2.35 ± 0.35, and that of their EBP talent/ competency was 1.51 ± 0.56 (on 0-3 Likert scale). In complete, 76.0% of physicians typically or generally utilized EBM in routine each day observe. The largest barrier stopping implementation was the various particular person variations in ailments (61.0%), adopted by a scarcity of funding from the hospital/division (39.8%), and a scarcity of affected person cooperation (37.4%). Chinese physicians in tertiary hospitals possessed expressed optimistic attitudes in direction of EBM; nevertheless, they solely retained a average stage of scientific proof competency.
Both a person issue (private curiosity) and organizational elements (workload, hospital requirement) had an impact on physicians’ attitudes and their EBP expertise. Management and organizational efforts, as well as to time devoted for EBP initiatives may assist scale back obstacles that stop EBP.

SARC-F Validation and SARC-F+EBM Derivation in Musculoskeletal Disease: The SPSS-OK Study.

To validate the SARC-F questionnaire for sarcopenia screening in musculoskeletal illness setting, and to assess enhancements in diagnostic accuracy by including “EBM” (aged and physique mass index info) to the SARC-F.Diagnostic accuracy examine.The heart concerned on this examine was situated in an city space of Kobe City, Japan. People with musculoskeletal illness within the knee, hip, or backbone who have been scheduled for surgical remedy have been included.Sarcopenia was evaluated utilizing the Asian Working Group for Sarcopenia (AWGS) and the European Working Group on Sarcopenia in Older People (EWGSOP2), which included bioimpedance, handgrip energy, and gait pace. Patients answered the SARC-F questionnaire and their physique mass index was measured. SARC-F and “EBM” info have been mixed into an authentic rating.
The sensitivities, specificities, and areas below the receiver working attribute curve (AUC) have been estimated and in contrast to determine sarcopenia.A complete of 959 sufferers have been included. Sarcopenia by AWGS standards was recognized in 36 (3.8%) sufferers. SARC-F had a sensitivity of 41.7% and specificity of 68.5%. SARC-F+EBM had a sensitivity of 77.8% and specificity of 69.6%, with substantial enchancment in sensitivity (P<0.001). The AUCs for SARC-F and SARC-F+EBM have been 0.557 (95% confidence interval [CI] 0.452-0.662) and 0.824 (95% CI 0.762-0.886), respectively (P<0.001).
A fish cytokine related to human IL-3, IL-5, and GM-CSF, induces development of eosinophil/basophil/mast-cell type (EBM) granulocytes.
Similar outcomes have been obtained when EWGSOP2 standards have been used because the reference normal.The SARC-F alone just isn’t ample for locating circumstances in musculoskeletal illness settings. SARC-F+EBM considerably improved the sensitivity and general diagnostic accuracy of the SARC-F for screening sarcopenia. SARC-F+EBM is probably helpful for screening sarcopenia in numerous ethnic and illness settings.

A comparative examine on the nanoindentation habits, put on resistance and in vitro biocompatibility of SLM manufactured CP-Ti and EBM manufactured Ti64 gyroid scaffolds.

The current examine investigates the nanoindentation habits, put on resistance and in vitro biocompatibility of SLM manufactured CP-Ti and EBM manufactured Ti64 gyroid scaffolds and the outcomes have been in contrast to these of casting CP-Ti. Both the SLM- and EBM manufactured scaffolds exhibited anisotropic properties with greater diminished modulus (up to 10%) and nanohardness (up to 30%) within the transverse course than these in constructing course. The put on resistance of scaffolds in transverse course was greater than these of in constructing course by up to ∼25% and ∼82% for SLM manufactured CP-Ti and EBM manufactured Ti64 scaffolds, respectively.
The SLM manufactured CP-Ti scaffolds displayed important enhancement in put on resistance over solid dense CP-Ti with 75% decrease imply worn peak throughout a nanowear take a look at. The coefficient of friction was various between 0.11 and 0.24 and exhibited a gentle imply worth of 0.15-0.18 for CP-Ti and Ti64 scaffolds, respectively. During in vitro cell tradition examine, CP-Ti scaffolds confirmed greater cell viability and cell adhesion density compared to Ti64 scaffolds for all unit cell sizes.
Moreover, cell adhesion density of scaffolds with smaller unit cell sizes (G2) are decrease than these of bigger unit cells (G3). SEM observations confirmed that each the internal area and surfaces of gyroid scaffolds offered an appropriate setting for cell migration, attachment and proliferation after cell tradition for 7 d. STATEMENT OF SIGNIFICANCE: It is crucial to consider the properties of EBM/SLM manufactured scaffolds and to decide whether or not they can meet the robust efficiency necessities of the biomedical trade.

FOLR CRISPR Knock Out Human OVCAR3-A1 Cell Line

T6229 1x10^6 cells / 1.0 ml Ask for price

MUC16 CRISPR Knock Out Human OVCAR3-A1 Cell Line

T6230 1x10^6 cells / 1.0 ml Ask for price

SIRT CRISPR Knock Out Human HepG2 Cell Line - Clone T3-6

T6804 1x10^6 cells / 1.0 ml Ask for price

mAdrb2 CRISPR Knock Out B16 Cell Line (T1-10)

T9775 1x10^6 cells / 1.0 ml Ask for price

PRSS21 CRISPR Knock Out Human OVCAR8 Cell Line - Clone T2 C8

T6811 1x10^6 cells / 1.0 ml Ask for price

NUAK2 CRISPR Knock Out 293T Cell Line - Clone T3 #41

T6801 1x10^6 cells / 1.0 ml Ask for price

TREM2 CRISPR Knock Out Human THP-1 Cell Line - Clone T1-9

T6810 1x10^6 cells / 1.0 ml Ask for price

PTPN6 CRISPR Knock Out Human THP-1 Cell Line - Clone T3-1

T6813 1x10^6 cells / 1.0 ml Ask for price

MSLN CRISPR Knock Out Human OVCAR3-A1 Cell Line - Clone T2 C4

T6820 1x10^6 cells / 1.0 ml Ask for price

NR2F6 CRISPR Knock Out Human Jurkat E6.1 Cell Line - Clone T3-10

T6822 1x10^6 cells / 1.0 ml Ask for price

hPIM1 CRISPR Knock Out SACC-83 Cell Line-T3-9

T9787 1x10^6 cells / 1.0 ml Ask for price

Aldh2 CRISPR Knock Out L929 Cell Line - Clone T3-20

T9692 1x10^6 cells / 1.0 ml Ask for price

Dcx CRISPR Knock Out C6 Cell Line (Rat) - Clone sg4-1

T9624 1x10^6 cells / 1.0 ml
EUR 3950

GLT1 CRISPR Knock Out Rat AST Cell Line - Clone T2-1

T9678 1x10^6 cells / 1.0 ml Ask for price

PTPN11 CRISPR Knock Out NIH/3T3 Cell Line - Clone T4-9

T6803 1x10^6 cells / 1.0 ml Ask for price

METTL3 CRISPR Knock Out Caki-1 Cell Line - Clone T2-16

T9623 1x10^6 cells / 1.0 ml
EUR 3950

Nacc1 CRISPR Knock Out ID8 Cell Line (Mouse) - Clone T1-23

T9627 1x10^6 cells / 1.0 ml Ask for price

osbpl2 CRISPR Knock Out NIH/3T3 Cell Line - T1 Polyclonal Pool

T9682 1x10^6 cells / 1.0 ml Ask for price

SEC61G CRISPR Knock Out HCC-LM3 Cell Line - Clone T2-17-5

T9746 1x10^6 cells / 1.0 ml Ask for price

Aifm1 CRISPR Knock Out Mouse L929 Cell Line - Clone T2-15-3

T9674 1x10^6 cells / 1.0 ml Ask for price

CARMIL1 CRISPR Knock Out Immortalized Human Gingival Fibroblast - hTERT Cell Line - T1-2

T6825 1x10^6 cells / 1.0 ml Ask for price

Cell line gene knock-in and knock-out

S301 -
EUR 15000

Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T3-3

T9529 1x10^6 cells/1.0ml
EUR 3950

Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T1-19

T9511 1x10^6 cells / 1.0 ml
EUR 3950

Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line

T3187 1x10^6 cells / 1.0 ml
EUR 3950

GFP-Human MDA-MB-435(MDA-MB-435)

ABC-RC211D 1 vial Ask for price
Description: Human MDA-MB-435 is a cell line isolated from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast. GFP-expressing MDA-MB-435 (GFP-MDA-MB-435) are selected from MDA-MB-435 cells after infected with lentiviruses expressing GFP with puromycin. The cells are shipped in a frozen vial with more than 5 x 105 cells/vial. Universal Full Growth Medium is recommended for cell culture and these cells have an average minimum population doubling levels > 20 when cultured following the detailed protocol described below.

CRISPRa (SAM) MDA-MB-231 Cell Line

78521 2 vials
EUR 2500
Description: This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR-associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express a construct consisting of P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag (from the MS2 coat protein), which is maintained with Hygromycin resistance. When these cells are transfected with an MS2 aptamer fused to a single guide RNA (sgRNA)targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the promoter in the genomic DNA by the MS2 aptamer and begin transcription, inducing high level expression of the desired protein._x000D_Cells were functionally validated by transfecting the cells with an sgRNA-MS2 targeting PD-1, resulting in high expression of PD-1.

Human MB knockout cell line

ABC-KH9065 1 vial Ask for price
Description: Human MB knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Human MB knockdown cell line

ABC-KD9065 1 vial Ask for price
Description: Human MB knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

PTPN6 Stable Knocked Out THP-1 Cell Line

T6216 1x10^6 cells / 1.0 ml
EUR 3950

MDA-MB-231 Cell Line

CL-0150 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

MDA-MB-453 Cell Line

CL-0152 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

MDA-MB-468 Cell Line

CL-0290 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

MDA-MB-415 Cell Line

CL-0382 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

MDA-MB-436 Cell Line

CL-0383 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

MDA-MB-361 Cell Line

CL-0553 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

MDA-MB-175VII Cell Line

CL-0381 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

Malondialdehyde (MDA) Modified Human Albumin

STA-210 100 µg
EUR 505.2
Description:
  • Malondialdehyde (MDA) Modified Human Albumin. 100 µg.
  • Provided at a concentration of 1.0 mg/mL

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Human CK-MB ELISA Kit (Creatine Kinase-MB)

PRB-5047 96 assays
EUR 686.4
Description: Our Human CK-MB ELISA Kit specifically detects the CK-MB form of Creatine Kinase in serum or plasma samples as well as in lysates. The kit does not detect the CK-MM or CK-BB isotypes. Each kit provides sufficient reagents for 96 wells including standards and unknown samples.

Human CK-MB ELISA Kit (Creatine Kinase-MB)

PRB-5047-5 5 x 96 assays
EUR 2739.6
Description: Our Human CK-MB ELISA Kit specifically detects the CK-MB form of Creatine Kinase in serum or plasma samples as well as in lysates. The kit does not detect the CK-MM or CK-BB isotypes. Each kit provides sufficient reagents for 96 wells including standards and unknown samples.

Malondialdehyde (MDA) Modified Human Apolipoprotein B-100

STA-211 100 µg
EUR 748.8
Description:
  • Malondialdehyde (MDA) Modified Human Apolipoprotein B-100. 100 µg.
  • Provided at a concentration of 1.0 mg/mL

MDA-BSA Control

STA-333 100 µg
EUR 386.4
Description:
  • MDA-BSA (Malondialdehyde-BSA) may be used as a positive control. 100 µg.
  • Provided at 1 mg/mL in 1X PBS

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)

AST-7001 1 Kit Ask for price
Description: 6 month

OxiSelect Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation)

STA-369 96 assays
EUR 936
Description: LDL, so-called "bad cholesterol", is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. Oxidation of LDL affects both the lipid and protein components of LDL.  The OxiSelect Human Oxidized LDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples. The kits contain a copper oxidized LDL standard against which unknown samples may be compared. This oxidized LDL assay kit is configured to selectively measure oxLDL in MDA-LDL.

OxiSelect Human Oxidized HDL ELISA Kit (MDA-HDL Quantitation)

STA-869 96 assays
EUR 936
Description: The OxiSelect Human Oxidized HDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxHDL in plasma, serum or other biological fluid samples. This oxidized HDL assay kit is configured to selectively measure MDA-modified HDL. The kit contains an MDA-HDL standard against which unknown samples may be compared. Note: For samples stored for more than one month, better results may be obtained with measurement of CML or HNE modifications, both of which are more stable than MDA.

Human NFkB Luciferase Reporter Cell Line- MDA-MB-231

ABC-RC0026 1 vial Ask for price
Description: Human NFkB Luciferase Reporter Cell Line- MDA-MB-231 is derived from Human breast cancer, and stably express firefly luciferase reporter gene under the control of NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

EBM 3'UTR GFP Stable Cell Line

TU056522 1.0 ml
EUR 2799.6

BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line

T6245 1x10^6 cells / 1.0 ml
EUR 3950

Human AR Luciferase Reporter Cell Line-MDA-MB-453

ABC-RC0005 1 vial Ask for price
Description: Human AR Luciferase Reporter Cell Line-MDA-MB-453 is derived from human breast cancer,and stably express firefly luciferase reporter gene under the control of the AR response element. This cell line is an ideal cellular model for monitoring the activation of Androgen Receptor Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human GR Luciferase Reporter Cell Line-MDA-MB-453

ABC-RC0015 1 vial Ask for price
Description: Human GR Luciferase Reporter Cell Line-MDA-MB-453 is derived from human breast cancer, and stably express firefly luciferase reporter gene under the control of the GR response element. This cell line is an ideal cellular model for monitoring the activation of Glucocorticoid Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

OxiSelect MDA Immunoblot Kit

STA-331 10 blots
EUR 664.8
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Immunoblot Kit provides a specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

TARGATT? Knock-in iPSC Generation (Master Cell Line)

AST-1100 1 vial of 1X10^6 cells Ask for price
Description: 6 month

Human CRISP1 knockout cell line

ABC-KH3569 1 vial Ask for price
Description: Human CRISP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP2 knockout cell line

ABC-KH3570 1 vial Ask for price
Description: Human CRISP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP3 knockout cell line

ABC-KH3571 1 vial Ask for price
Description: Human CRISP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

EBM 3'UTR Luciferase Stable Cell Line

TU006522 1.0 ml
EUR 2799.6

PTPRA CRISPR Knockout Immortalized Human Lung Fibroblast Cell Line

T6827 1x10^6 cells / 1.0 ml Ask for price

Human CRISPLD1 knockout cell line

ABC-KH3572 1 vial Ask for price
Description: Human CRISPLD1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISPLD2 knockout cell line

ABC-KH3573 1 vial Ask for price
Description: Human CRISPLD2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP1 knockdown cell line

ABC-KD3569 1 vial Ask for price
Description: Human CRISP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISP2 knockdown cell line

ABC-KD3570 1 vial Ask for price
Description: Human CRISP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISP3 knockdown cell line

ABC-KD3571 1 vial Ask for price
Description: Human CRISP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 1 Kit Ask for price
Description: 12 month

Acan CRISPR Knockout RCS Cell Line (Rat)

T3000 1x10^6 cells / 1.0 ml Ask for price

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 1 Kit Ask for price
Description: 12 month

Human CRISPLD1 knockdown cell line

ABC-KD3572 1 vial Ask for price
Description: Human CRISPLD1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISPLD2 knockdown cell line

ABC-KD3573 1 vial Ask for price
Description: Human CRISPLD2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

OxiSelect TBARS Assay Kit (MDA Quantitation)

STA-330 200 assays
EUR 790.8
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect TBARS Assay Kit (MDA Quantitation)

STA-330-5 5 x 200 assays
EUR 3205.2
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect MDA Adduct Competitive ELISA Kit

STA-832 96 assays
EUR 957.6
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect MDA Adduct Competitive ELISA Kit

STA-832-5 5 x 96 assays
EUR 3888
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

MDA-MB-231 / GFP (Bsd) Stable Cell Line

SC040-Bsd 2 x 106 cell/ml x 1ml
EUR 1452
Description: GFP expression stable cell line in MDA-MB-231 cells with Blasticidin resistance

MDA-MB-231 / RFP (Bsd) Stable Cell Line

SC057-Bsd 2 x 106 cell/ml x 1ml
EUR 1452
Description: RFP expression stable cell line in MDA-MB-231 cells with Blasticidin resistance

MDA-MB-231 / GFP (Puro) Stable Cell Line

SC040-Puro 2 x 106 cell/ml x 1ml
EUR 1452
Description: GFP expression stable cell line in MDA-MB-231 cells with Puromycin resistance

Goat Anti-Malondialdehyde (MDA) Polyclonal Antibody

STA-031 100 µg
EUR 609.6
Description: Goat Anti-Malondialdehyde Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

Rabbit Anti-Malondialdehyde (MDA) Polyclonal Antibody

STA-032 100 µg
EUR 609.6
Description: Rabbit Anti-Malondialdehyde Polyclonal Antibody is Affinity purified and suitable for Western blot or ELISA. Suitable for use with any species. 100 µg.

MDA-MB-231 / Luciferase (Bsd) Stable Cell Line

SC059-Bsd 2 x 106 cell/ml x 1ml
EUR 1800
Description: Luciferase (firefly) expression stable cell line in MDA-MB-231 cells with Blasticidin resistance

MDA-MB-231 / Luciferase (Puro) Stable Cell Line

SC059-Puro 2 x 106 cell/ml x 1ml
EUR 1800
Description: Luciferase (firefly) expression stable cell line in MDA-MB-231 cells with Puromycin resistance

Luciferase(Bsd) Report Cell Line-MDA-MB-231

ABC-RC157D 1 vial Ask for price
Description: Transformed from human breast cancer cell line, derived from metastatic breast cancer, mammary gland epithelial cells., MDA-MB-231 adhesive cell using lentivirus expressing firefly luciferase gene. The Luciferase was expressed under the enhanced CMV promoter. The Blasticidin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

GFAT1 CRISPR Stable Knockout H1299 Cell Line

T6199 1x10^6 cells / 1.0 ml
EUR 3950

Luciferase(Puro) Report Cell Line-MDA-MB-231

ABC-RC158D 1 vial Ask for price
Description: Transformed from human breast cancer cell line, derived from metastatic breast cancer, mammary gland epithelial cells., MDA-MB-231 adhesive cell using lentivirus expressing firefly luciferase gene. The Luciferase was expressed under the enhanced CMV promoter. The Puromycin marker was expressed under Rsv promoter. The cell line demonstrates high level of bioluminescence signal via firefly luciferase assay.

Malondialdehyde (MDA) Modified Low Density Lipoprotein (LDL)

STA-212 100 µg
EUR 540
Description:
  • Malondialdehyde (MDA) Modified Low Density Lipoprotein (LDL). 100 µg.
  • Provided at a concentration of 1.0 mg/mL

EDC4 CRISPR Stable Knockout HEK293T Cell Line

T6241 1x10^6 cells / 1.0 ml
EUR 3950

DJ-1 CRISPR Stable Knockout HeLa Cell Line

T6181 1x10^6 cells / 1.0 ml
EUR 3950

PD-L1 CRISPR Stable Knockout CT26 Cell Line

T6237 1x10^6 cells / 1.0 ml
EUR 3950

PDPN CRISPR Stable Knockout COS-7 Cell Line

T6039 1x10^6 cells / 1.0 ml
EUR 3950

PD-L1 CRISPR Stable Knockout MC38 Cell Line

T6149 1x10^6 cells / 1.0 ml
EUR 3950

LIMD1-/- CRISPR Stable Knockout HeLa Cell Line

T6357 1x10^6 cells / 1.0 ml
EUR 3950

OxiSelect TBARS Assay Kit (MDA Quantitation), Trial Size

STA-330-T 20 assays
EUR 408
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect MDA Adduct Competitive ELISA Kit, Trial Size

STA-832-T 32 assays
EUR 505.2
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

hCETP shRNA (Target C) Knock Down THP-1 Cell Line-Polyclone

T9789 1x10^6 cells / 1.0 ml Ask for price

SLC35A1 CRISPR Stable Knockout CHO-S Cell Line

T6040 1x10^6 cells / 1.0 ml
EUR 3950

CXCL1 & CXCL8 CRISPR Double Knockout L3.6pl Cell Line

T6047 1x10^6 cells / 1.0 ml Ask for price

Human MDA-MB-361 Cell Lysate

IHUMDAMB361TL100UG each
EUR 245
Description: Human MDA-MB-361 Cell Lysate

AXL CRISPR Stable Knockout HT1080 (1-IV) Cell Line

T6232 1x10^6 cells / 1.0 ml
EUR 3950

AXL CRISPR Stable Knockout HT1080 (3-II) Cell Line

T6233 1x10^6 cells / 1.0 ml
EUR 3950

MDA-MB-231 / Luciferase-2A-RFP Stable Cell Line

SC041 2 x 106 cell/ml x 1ml
EUR 2670
Description: Firefly luciferase and GFP co-expression stable cell line in MDA-MB-231 cells with Blasticidin resistance

MDA-MB-231 / Luciferase-2A-GFP Stable Cell Line

SC044 2 x 106 cell/ml x 1ml
EUR 2670
Description: Luciferase (firefry) and GFP co-expression stable cell line in MDA-MB-231 human breast cancer cell line with Puromycin marker.

Human CRIM1 knockout cell line

ABC-KH3563 1 vial Ask for price
Description: Human CRIM1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP1 knockout cell line

ABC-KH3564 1 vial Ask for price
Description: Human CRIP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP2 knockout cell line

ABC-KH3565 1 vial Ask for price
Description: Human CRIP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP3 knockout cell line

ABC-KH3566 1 vial Ask for price
Description: Human CRIP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIPT knockout cell line

ABC-KH3568 1 vial Ask for price
Description: Human CRIPT knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Luciferase-2A-GFP Report Cell Line-MDA-MB-231

ABC-RC159D 1 vial Ask for price
Description: Transformed from MDA-MB-231 cells by lentivirus, expressing the firefly luciferase gene III and enhanced GFP fluorescent marker. The Luciferase and GFP was co-expressed under the same suCMV promoter as separated two proteins(not as fusion). The Puromycin marker was expressed under Rsv promoter.Each cell demonstrated strong GFP Fluorescent signal and high level of luminescence signal via luciferase assay. Note: this is pooled stable ell population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells.

Luciferase-2A-RFP Report Cell Line-MDA-MB-231

ABC-RC160D 1 vial Ask for price
Description: Transformed from MDA-MB-231 cells by lentivirus, expressing the firefly luciferase gene(3) and enhanced RFP fluorescent marker.The Luciferase and RFP was co-expressed under the same suCMV promoter as separated two proteins(not as fusion). The Blasticidin marker was expressed under Rsv promoter.Each cell demonstrated strong RFP Fluorescent signal and high level of luminescence signal via luciferase assay.

Human CRIPAK knockout cell line

ABC-KH3567 1 vial Ask for price
Description: Human CRIPAK knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.
In this examine, nanoindentation and nanowear properties of SLM manufactured CP-Ti and EBM manufactured Ti64 gyroid scaffolds with completely different unit cell sizes and pattern orientations have been evaluated and in contrast to solid dense CP-Ti samples. Moreover, the in vitro biocompatibility of the scaffolds was assessed and in contrast to one another. To our greatest of information, this type of examine on EBM/SLM manufactured CP-Ti and Ti64 scaffolds haven’t been reported, to date.